Table 2. Effect of metal ions and chemical reagents on the activity of the recombinant enzymes.
| Chemicals | Relative activity (%) |
|||
|---|---|---|---|---|
| xyn10A | xyn11A | xyn10B | xyn11B | |
| Control | 100.0± 0.4 | 100.0 ± 3.7 | 100.0 ± 1.6 | 100.0 ± 0.6 |
| Ca2+ | 96.1 ± 0.8 | 118.9 ± 1.7 | 97.6 ± 0.7 | 100.1 ± 3.4 |
| Cd2+ | 18.5 ± 1.0 | 116.2 ± 2.4 | 95.6 ± 0.7 | 78.9 ± 4.2 |
| Cr3+ | 77.7 ± 2.8 | 110.9 ± 3.1 | 79.7 ± 0.8 | 79.4 ± 0.4 |
| Co2+ | 73.6 ± 2.2 | 124.9 ± 2.5 | 37.2 ± 0.3 | 81.7 ± 1.6 |
| Cu2+ | 3.6 ± 0.4 | 2.3 ± 0.16 | 3.9 ± 1.2 | 41.6 ± 2.6 |
| Ba2+ | 95.7 ± 0.1 | 120.8 ± 2.6 | 105.1 ± 6.0 | 100.1 ± 2.4 |
| Mg2+ | 93.9 ± 0.5 | 127.6 ± 2.0 | 94.4 ± 4.4 | 101.8 ± 2.7 |
| Mn2+ | 91.6 ± 1.4 | 108.8 ± 2.8 | 98.3 ± 3.0 | 100.1 ± 2.7 |
| Ni2+ | 77.1 ± 2.0 | 116.1 ± 2.5 | 93.9 ± 1.1 | 79.1 ± 3.1 |
| Li+ | 85.1 ± 0.7 | 119.4 ± 2.3 | 93.7 ± 3.2 | 89.6 ± 3.9 |
| Fe2+ | 88.4 ± 1.5 | 115.5 ± 3.1 | 146.4 ± 3.1 | 101.3 ± 2.5 |
| Fe3+ | 0.8 ± 0.02 | 22.8 ± 1.0 | 8.7 ± 0.2 | 33.0 ± 0.5 |
| Hg2+ | 6.3 ± 0.7 | 24.4 ± 2.5 | 0.0 ± 0.0 | 1.4 ± 0.4 |
| 1 mM β-Mercaptoethanol | 99.2 ± 0.9 | 41.7 ± 0.1 | 98.5 ± 2.0 | 101.0 ± 5.3 |
| 1 mM EDTA | 99.2 ± 1.0 | 7.0 ± 0.04 | 87.2 ± 1.5 | 79.7 ± 1.8 |
| 1 mM DTT | 101.6 ± 1.4 | 100.2 ± 1.2 | 100.0 ± 1.3 | 98.3 ± 2.6 |
| 0.1% Triton | 99.7 ± 1.6 | 100.0 ± 1.1 | 89.2 ± 1.0 | 88.8 ± 2.9 |
| 0.1% Tween-20 | 99.2 ± 1.6 | 100.8 ± 1.2 | 92.2 ± 2.0 | 104.6 ± 7.6 |
| 0.1% SDS | 72.3 ± 9.1 | 23.9 ± 1.8 | 73.2 ± 4.8 | 3.5 ± 0.3 |