Table 3. Comparison of kinetic values of four recombinant xylanases with various xylans as substrate.
| Enzyme |
Km (mg/ml) |
Vmax(U/mg) |
kcat (1/s) |
kcat/Km (ml/mg/s) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ox | Bi | Be | Ox | Bi | Be | Ox | Bi | Be | Ox | Bi | Be | |
| xyn10A | 5.4 ± 0.4 Ac | 3.3 ± 0.3 Bb | 3.0 ± 0.3 Bb | 181.3 ± 12.5 Bd | 144.5± 8.1 Cc | 247.4 ± 13.8 Ac | 103.4 ± 7.1 Bc | 82.3 ± 4.6 Cc | 141.0 ± 7.9 Ac | 19.2 ± 0.1 Ac | 24.8 ± 0.6 Bc | 47.2 ± 2.4 Ac |
| xyn11A | 7.9 ± 0.8 Ab | 2.8 ± 0.2 Bb | 3.0 ±0.1 Bb | 500.1 ± 52.5 Ac | 115.2 ±13.7 Bc | 244.8 ± 9.4 Bc | 177.5 ± 18.6 Ac | 40.9 ± 4.9 Cc | 86.9 ± 3.4 Bc | 22.4 ± 0.8 Bc | 14.6 ± 0.6 Cc | 28.7 ± 0.2 Ac |
| xyn10B | 1.8 ± 0.0 Ad | 1.3 ± 0.1 Bc | 1.0 ± 0.1 Cc | 1397.8 ± 38.2 Ab | 1140.6 ± 30.4 Bb | 914.4 ± 31 Cb | 964.5 ± 26.4 Ab | 787.0 ± 20.9 Bb | 631.0 ± 21.4 Cb | 535.5 ± 8.6 Ba | 626.4 ± 17.9 Aa | 648.1 ± 27.0 Aa |
| xyn11B | 11.6 ± 1.2 Aa | 7.5 ± 0.7 Ba | 4.7 ± 0.8 Ca | 4604.7 ± 469.6 Aa | 3486.8 ± 276.1 Aa | 4426.2 ± 397.0 Aa | 2264.0 ± 230.9 Aa | 1714.3 ± 135.8 Aa | 2176.2 ± 195.2 Aa | 195.9 ± 0.7 Bb | 229.1 ± 2.1 Bb | 467.8 ± 36.7 Ab |
Ox: oat spelt xylan, Bi: birchwood xylan, Be: beechwood xylan. A, B, C: per substrate, values with the same letter in one row are not significantly different from each other (P-value <0.05).
a, b, c, d: per enzyme, values with the same letter in one column are not significantly different from each other (P-value <0.05).