Table 2. Characteristics of a complete collection of overlapping segmental aneuploids of chromosomes I to XVI.
| Region | Strain name | Duplicated regiona | Plasmid templateb | Duplication length (kb) | Number of genes | Transformants (n) | Proportion of desired karyotypec | % Mitotic stability |
|---|---|---|---|---|---|---|---|---|
| C1-1 | ScDup(C1-1) | Chr. I 1–100,705 | p3122 | 100 | 65 | 7 | 71% (5/7) | 98% |
| C1-2 | ScDup(C1-2) | Chr. I 99,603–230,218 | p3008 | 130 | 85 | 16 | 50% (8/16) | 99% |
| C2-1 | ScDup(C2-1) | Chr. II 1–202,750 | p3122 | 200 | 137 | 70 | 67% (6/9) | 98.57% |
| C2-2 | ScDup(C2-2) | Chr. II 201,029–401,862 | p3008, p3009 | 200 | 128 | 6 | 17% (1/6) | 100% |
| C2-3 | ScDup(C2-3) | Chr. II 400,204–600,988 | p3009, p3122 | 200 | 124 | 25 | 5% (1/22) | 99.79% |
| C2-4 | ScDup(C2-4) | Chr. II 599,536–813,184 | p3122 | 213 | 142 | 29 | 100% (9/9) | 100% |
| C3-1 | ScDup(C3-1) | Chr. III 1–158,020 | p3008 | 158 | 139 | 4 | 75% (3/4) | 99% |
| C3-2 | ScDup(C3-2) | Chr. III 157,543–316,620 | p3122 | 159 | 110 | 5 | 20% (1/5) | 100% |
| C4-1 | ScDup(C4-1) | Chr. IV 1–200,732 | p3122 | 200 | 119 | 56 | 78% (7/9) | 97.70% |
| C4-2# | ScDup(C4-2) | Chr. IV 198,996–401,638 | p3009, p3122 | 200 | 128 | 219 | 0% (0/219) | ND |
| C4-3 | ScDup(C4-3) | Chr. IV 399,987–600,688 | p3008, p3009 | 200 | 140 | 5 | 20% (1/5) | 100% |
| C4-4# | ScDup(C4-4) | Chr. IV 599,793–795,723 | p3009, p3122 | 200 | 114 | 134 | 0% (0/134) | ND |
| C4-5# | ScDup(C4-5) | Chr. IV 795,193–1,000,877 | p3009, p3122 | 200 | 133 | 22 | 0% (0/22) | ND |
| C4-6 | ScDup(C4-6) | Chr. IV 999,134–1,199,697 | p3009, p3122 | 200 | 121 | 13 | 8% (1/13) | 99.56% |
| C4-7# | ScDup(C4-7) | Chr. IV 1,198,183–1,402,247 | p3009, p3122 | 200 | 134 | 27 | 0%(0/27) | ND |
| C4-8 | ScDup(C4-8) | Chr. IV 1,400,770–1,531,933 | p3122 | 130 | 89 | 41 | 89% (8/9) | 100% |
| C5-1 | ScDup(C5-1) | Chr. V 1–199,519 | p3008 | 200 | 146 | 17 | 80% (8/10) | 100% |
| C5-2 | ScDup(C5-2) | Chr. V 197,812–400,060 | p3009, p3122 | 200 | 143 | 5 | 20% (1/5) | 99.77% |
| C5-3 | ScDup(C5-3) | Chr. V 398,496–576,874 | p3122 | 177 | 127 | 5 | 20% (1/5) | 100% |
| C6-1# | ScDup(C6-1) | Chr. VI 1–98,498 | p3122 | 100 | 57 | 24 | 0% (0/24) | ND |
| C6-2 | ScDup(C6-2) | Chr. VI 98,213–270,161 | p3008 | 171 | 128 | 8 | 50% (4/8) | 100% |
| C7-1 | ScDup(C7-1) | Chr. VII 1–201,147 | p3122 | 200 | 125 | 14 | 57% (8/14) | 100% |
| C7-2 | ScDup(C7-2) | Chr. VII 199,564–398,642 | p3009, p3122 | 200 | 128 | 3 | 67% (2/3) | 97.45% |
| C7-3 | ScDup(C7-3) | Chr. VII 397,621–599,626 | p3008, p3009 | 200 | 154 | 15 | 7% (1/15) | 100% |
| C7-4# | ScDup(C7-4) | Chr. VII 598,443–801,057 | p3009, p3122 | 200 | 133 | 156 | 0% (0/156) | ND |
| C7-5 | ScDup(C7-5) | Chr. VII 799,553–1,090,940 | p3122 | 290 | 181 | 10 | 60% (6/10) | 100% |
| C8-1 | ScDup(C8-1) | Chr. VIII 1–202,241 | p3008 | 200 | 146 | 22 | 44% (4/9) | 100% |
| C8-2# | ScDup(C8-2) | Chr. VIII 203,559–401,907 | p3009, p3122 | 200 | 140 | 72 | 0% (0/72) | ND |
| C8-3 | ScDup(C8-3) | Chr. VIII 400,443–562,643 | p3122 | 160 | 99 | 27 | 44% (4/9) | 100% |
| C9-1 | ScDup(C9-1) | Chr. IX 1–203,042 | p3122 | 200 | 116 | 31 | 78% (7/9) | 99.68% |
| C9-2 | ScDup(C9-2) | Chr. IX 201,284–439,888 | p3008 | 240 | 175 | 11 | 56% (5/9) | 100% |
| C10-1 | ScDup(C10-1) | Chr. X 1–195,892 | p3122 | 200 | 131 | 7 | 29% (2/7) | 100% |
| C10-2 | ScDup(C10-2) | Chr. X 195,298–403,454 | p3009, p3122 | 200 | 130 | 18 | 11% (2/18) | 100% |
| C10-3 | ScDup(C10-3) | Chr. X 401,881–599,357 | p3008, p3009 | 200 | 142 | 6 | 17% (1/6) | 100% |
| C10-4 | ScDup(C10-4) | Chr. X 597,731–745,751 | p3122 | 150 | 87 | 12 | 67% (8/12) | 100% |
| C11-1 | ScDup(C11-1) | Chr. XI 1–201,168 | p3009, p3122 | 200 | 116 | 6 | 50% (3/6) | 100% |
| C11-2# | ScDup(C11-2) | Chr. XI 199,892–399,750 | p3009, p3122 | 200 | 133 | 202 | 0% (0/100) | ND |
| C11-3 | ScDup(C11-3) | Chr. XI 397,819–666,816 | p3008 | 267 | 153 | 58 | 90% (9/10) | 100% |
| C12-1 | ScDup(C12-1) | Chr. XII 1–251,980 | p3008 | 250 | 146 | 20 | 10% (2/20) | 99.89% |
| C12-2 | ScDup(C12-2) | Chr. XII 250,272–450,039 | p3009, p3122 | 200 | 117 | 9 | 11% (1/9) | 100% |
| C12-3 | ScDup(C12-3) | Chr. XII 490,862–692,029 | p3009, p3122 | 200 | 140 | 11 | 9% (1/11) | 100% |
| C12-4 | ScDup(C12-4) | Chr. XII 690,555–885,764 | p3009, p3122 | 200 | 139 | 34 | 10% (1/10) | 99.03% |
| C12-5 | ScDup(C12-5) | Chr. XII 884,258–1,078,177 | p3122 | 200 | 115 | 73 | 70% (7/10) | 100% |
| C13-1 | ScDup(C13-1) | Chr. XIII 1–204,690 | p3122 | 200 | 130 | 5 | 20% (1/5) | 100% |
| C13-2 | ScDup(C13-2) | Chr. XIII 203,398–402,207 | p3008, p3009 | 200 | 141 | 1 | 100% (1/1) | 99.04% |
| C13-3 | ScDup(C13-3) | Chr. XIII 400,538–600,143 | p3009, p3122 | 200 | 133 | 33 | 3% (1/29) | 82.02% |
| C13-4 | ScDup(C13-4) | Chr. XIII 598,338–798,915 | p3009, p3122 | 200 | 120 | 11 | 9% (1/11) | 100% |
| C13-5 | ScDup(C13-5) | Chr. XIII 797,512–924,441 | p3122 | 120 | 83 | 29 | 60% (6/10) | 98.91% |
| C14-1 | ScDup(C14-1) | Chr. XIV 1–200,971 | p3122 | 200 | 122 | 21 | 43% (9/21) | 96.67% |
| C14-2# | ScDup(C14-2) | Chr. XIV 199,575–403,514 | p3009, p3122 | 200 | 132 | 152 | 0% (0/152) | ND |
| C14-3 | ScDup(C14-3) | Chr. XIV 401,690–598,530 | p3009, p3122 | 200 | 130 | 29 | 3% (1/29) | 99.08% |
| C14-4 | ScDup(C14-4) | Chr. XIV 597,394–784,333 | p3008 | 184 | 118 | 7 | 14% (1/7) | 100% |
| C15-1 | ScDup(C15-1) | Chr. XV 1–201,315 | p3122 | 200 | 125 | 20 | 56% (5/9) | 99.87% |
| C15-2 | ScDup(C15-2) | Chr. XV 199,377–401,104 | p3008, p3009 | 200 | 135 | 17 | 6% (1/17) | 100% |
| C15-3 | ScDup(C15-3) | Chr. XV 399,345–603,357 | p3009, p3122 | 200 | 128 | 16 | 6% (1/16) | 99% |
| C15-4 | ScDup(C15-4) | Chr. XV 601,731–801,721 | p3009, p3122 | 200 | 134 | 9 | 11% (1/9) | 84.76% |
| C15-5 | ScDup(C15-5) | Chr. XV 799,959–1,091,289 | p3122 | 290 | 176 | 67 | 56% (5/9) | 99.45% |
| C16-1 | ScDup(C16-1) | Chr. XVI 1–198,780 | p3122 | 200 | 124 | 39 | 44% (4/9) | 99.71% |
| C16-2 | ScDup(C16-2) | Chr. XVI 198,090–399,110 | p3009, p3122 | 200 | 116 | 6 | 17% (1/6) | 100% |
| C16-3 | ScDup(C16-3) | Chr. XVI 397,495–597,301 | p3008, p3009 | 200 | 124 | 8 | 13% (1/8) | 100% |
| C16-4 | ScDup(C16-4) | Chr. XVI 595,746–799,875 | p3009, p3122 | 200 | 136 | 6 | 17% (1/6) | 99.76% |
| C16-5 | ScDup(C16-5) | Chr. XVI 798,248–948,066 | p3122 | 148 | 112 | 46 | 26% (5/19) | 100% |
| YCp50 (7.8 kb) | – | 85% | ||||||
aChr. N x-y: Chr. N represents chromosome number, x represents first nucleotide number of chromosomal region and y represents last nucleotide number of chromosomal region.
bp3009 was used to amplify the CgHIS3 cassette, p3122 was used to amplify the CEN4-CgLEU2 cassette, p3008 was used to amplify the CgLEU2 cassette and YCp50 was a URA3 centromeric plasmid whose length was 7.8 kb.
cProportion of desired karyotype in analyzed transformants (number of segmental aneuploids/number of candidate transformants that were analyzed for karyotype)
#means region that could not be duplicated.
ND means not determined