Figure 3.7.

Stable cell lines developed to generate lentiviral vectors. Transgene, envelope, and packaging cassettes introduced into vector’s producer cells by stable transfection following by selection. Tet-inducible system is commonly employed to circumvent the toxicity associated with the viral proteins. Tetracycline transactivator, ttA can be expressed from the same cassette (transgene cassette) as does a transgene.