Figure 5.
The induction of γH2AX in MCF-10A cells by atrazine. (A) Time course of atrazine-induced γH2AX. MCF-10A cells (3 × 105) treated with 0.1 μg/mL atrazine for indicated hours and analyzed by Western blotting; (B) dose-response of atrazine-induced γH2AX. MCF-10A cells (3 × 105) were exposed to the indicated concentrations of atrazine for 1 h and analyzed by Western blotting. For each blot, the resulting fold-change of atrazine-mediated phospho-H2AX (Ser139) expression over DMSO untreated controls is provided in the corresponding histogram (n = 3) with the signal normalized to actin as a loading control; (C) The representative images showing γH2AX foci induced by 0.1 μg/mL of atrazine for indicated hours. Phospho-H2AX antibody was indirectly labeled with Alexa Fluor 488 secondary antibody (green), and cells were mounted with VECTASHIELD Mounting Medium with DAPI (purple). The images were captured using a Carl Zeiss confocal microscopy using the same exposure time. * p < 0.05, ** p < 0.01 vs. controls.