Figure 5.

Validation of proteomic data by immunoblot analysis. Five proteins identified by 2-DE analysis were confirmed by immunoblot analysis using pooled as well as individual samples of WAT. Data are representative of three independent experiments. Image master 2-DE software version 4.95 was used to obtain the band density, and the relative intensity (%) values of proteins were normalized by β-actin levels. Statistical significance was determined by Student’s t-test, where p values are ^† p < 0.05 and ^†† p < 0.01.