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. 2015 Jun 26;16(7):14511–14525. doi: 10.3390/ijms160714511

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression and knockdown of miR-21 influenced the DOX-induced cytotoxicity in H9C2 cells. (A) The levels of miR-21 in H9C2 cells transfected with rno-miR-21 mimics/inhibitors/scrambled controls were measured by qRT-PCR. *, p < 0.05, compared to that of cells treated with scramble, n = 3; **, p < 0.01, compared to that of cells transfected with scramble, n = 3; (B) Apoptosis induced by DOX (1 µM for 24 h) in H9C2 cells transfected with miR-21 mimics/inhibitors. *, p < 0.05, compared to that of cells transfected with scramble, n = 3; **, p < 0.01, compared to that of cells transfected with scramble, n = 3; ^#, p < 0.05, compared to that of cells transfected with scramble + DOX, n = 3; (C) Cell viability; and (D) Activity of lactate dehydrogenase. *, p < 0.05, compared to that of cells transfected with scramble, n = 6; **, p < 0.01, compared to that of cells transfected with scramble, n = 6; ^#, p < 0.05, compared to that of cells transfected with scramble + DOX, n = 6; ^##, compared to that of cells transfected with scramble + DOX, p < 0.01, n = 6.

Overexpression and knockdown of miR-21 influenced the DOX-induced cytotoxicity in H9C2 cells. (A) The levels of miR-21 in H9C2 cells transfected with rno-miR-21 mimics/inhibitors/scrambled controls were measured by qRT-PCR. *, p < 0.05, compared to that of cells treated with scramble, n = 3; **, p < 0.01, compared to that of cells transfected with scramble, n = 3; (B) Apoptosis induced by DOX (1 µM for 24 h) in H9C2 cells transfected with miR-21 mimics/inhibitors. *, p < 0.05, compared to that of cells transfected with scramble, n = 3; **, p < 0.01, compared to that of cells transfected with scramble, n = 3; ^#, p < 0.05, compared to that of cells transfected with scramble + DOX, n = 3; (C) Cell viability; and (D) Activity of lactate dehydrogenase. *, p < 0.05, compared to that of cells transfected with scramble, n = 6; **, p < 0.01, compared to that of cells transfected with scramble, n = 6; ^#, p < 0.05, compared to that of cells transfected with scramble + DOX, n = 6; ^##, compared to that of cells transfected with scramble + DOX, p < 0.01, n = 6.