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. 2015 Jun 26;16(7):14526–14539. doi: 10.3390/ijms160714526

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Blockage of eriodictyol-induced HO-1 protein expression by an ERK inhibitor. (A) Cells were pre-treated with increasing doses of PD 98059 (an ERK inhibitor) for 1 h prior to the treatment of eriodictyol 10 μM; (B) cell lysates were immunoblotted with antibodies against the phosphorylated form of ERK1/2 and total ERK; and (C) transient transfection of cells with increasing doses of ERK siRNA (20 and 30 nM) inhibited the induction of HO-1 protein expression by eriodictyol 10 μM. Western blots representative of three independent experiments are shown: C, untreated cells; +, treated with eriodictyol only; black line, treated with 10 µM eridictyol; , dose increasing.

Inline graphic — Blockage of eriodictyol-induced HO-1 protein expression by an ERK inhibitor. (A) Cells were pre-treated with increasing doses of PD 98059 (an ERK inhibitor) for 1 h prior to the treatment of eriodictyol 10 μM; (B) cell lysates were immunoblotted with antibodies against the phosphorylated form of ERK1/2 and total ERK; and (C) transient transfection of cells with increasing doses of ERK siRNA (20 and 30 nM) inhibited the induction of HO-1 protein expression by eriodictyol 10 μM. Western blots representative of three independent experiments are shown: C, untreated cells; +, treated with eriodictyol only; black line, treated with 10 µM eridictyol; , dose increasing.