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. 2015 Jun 30;16(7):14717–14736. doi: 10.3390/ijms160714717

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Accumulation of three different types of storage proteins in Glb-RNAi seeds. (a) Ground seed powder (50 mg) was fractionated based on solvent solubility, and the three fractionated extracts were resolved on an sodium dodecyl sulfate-polyacrylamide gel and stained in Coomasie Brilliant Blue (CBB) staining solution. arrows, prolamins; asterisk, globulins; arrow heads, glutelins; (b) The glutelin-containing extracts were used for an immunoblotting analysis. The immune signals recognized by the anti-glutelin antibodies were detected by a luminescent image analyzer (LAS-4000, Fujifilm). A, wild type; B, Glb-RNAi 1–6; C, Glb-RNAi 1–9.