Figure 4.

Extraction patterns of storage proteins from wild type (WT) and Glb-RNAi lines. (a) Proteins isolated in the absence (S) or presence (P) of β-ME were resolved on an SDS-PAGE gel and stained with CBB solution. M, unstained protein standard marker (Bio-Rad, #161-0363); *, globulins; (b) Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and antibodies directed against various glutelins were used to detect the accumulated glutelin acidic proteins. CBB-stained protein band at approximately 80 kDa indicates the internal loading control. S, supernatant; P, pellet.