Figure 5.

Isoelectric focusing and SDS-PAGE analyses of wild type (WT) and Glb-RNAi seeds. Equal amounts (20 mg) of seed powder from WT and Glb-RNAi transformant seeds were resolved in SDS-urea buffer and the total proteins were separated with 2-DE, as described in the Methods. The intensities of the labeled protein spots (shown inside the red circles) were lower in the protein extract from the transformants compared with those from WT extract. The CBB-stained 2-D gels were photographed and the images were analyzed with ImageJ. PI, isoelectric point.