Table 1.

Summary of rate constants measured for formation of binary hAgo2-guide, AaAgo-guide and hAgo2-PAZ9-guide complexes. Dissociation constants calculated from association and dissociation constants are displayed. Cartoons are based on hAgo2 X-ray structures with individual domains coloured. The guide substrate is depicted in blue with the 5′-end indicated by the phosphate group. Relative positions of the guide substrate are indicated. n.d. = values not determined in this study.

Collision Complex				5′-End Binding			3′-End Binding
Ago Protein	k1_bin (M−1·s−1)	k-1_bin (s−1)	k 2_bin (s−1)	k-2_bin (s−1)	k3_bin (s−1)	k-3_bin (s−1)	KD_bin (nM)	Reference
wt hAgo2	0.6 × 108	6.2	0.26	0.17	0.012	0.007	37	[46]
wt hAgo2	1.2 × 105	n.d.	n.d.	n.d.	n.d.	0.007	57	[47]
AaAgo	n.d.	n.d.	n.d.	n.d.	n.d.	0.004	10	[4,48]
hAgo2-PAZ9	0.2 × 108	7.8	0.18	0.024	–	–	49.5	[46]