Table 4.

Binding affinities of different Ago-guide binary complexes for various target substrates.

Substrate	KD (nM)	Reference
hAgo2
21-Mer RNA	0.2	[46]
19-Mer RNA	204	[47]
20-Mer RNA	104	[47]
29-Mer RNA	43	[47]
Sod1-RNA (complementarity for guide nt 2–7)	20	[39]
Sod1-RNA (complementarity for guide nt 2–8)	1.9	[39]
Sod1-RNA (complementarity for guide nt 2–9)	4.0	[39]
Sod1-RNA (complementarity for guide nt 2–10)	2.4	[39]
Fly Ago2
21-Mer RNA	0.004	[69]
Mouse Ago2
21-Mer RNA	0.02	[69]