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. 2015 Jul 3;16(7):15118–15135. doi: 10.3390/ijms160715118

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Proteoglycan analysis of scaffolds. (A) Fixed acellular, chondrocyte-, and NP-seeded ABS and PLA scaffolds were stained with safranin-O/fast green after 21 days’ culture. Representative images (n = 4) showing that both chondrocyte- and NP-seeded ABS and PLA scaffolds retained intense safranin-O staining. Ruler ticks represent millimeters; (B) DMMB assay for quantifying sulfated GAG in total extract per scaffold (in 1 mL); (C) DNA content was quantified by HOECHST 33258 assay per scaffold; (D) The sGAG/DNA ratio was determined. Error bars represent ± SD. * indicates p < 0.05.