Table 1.

Kinetic parameters for the CO₂ hydration reaction catalyzed by thermostable CAs. Data on hCA II have been added for comparison.

Enzyme	Class	kcat (s−1)	kcat/KM (M−1·s−1)	Ref.
hCA II	α	1.40 × 106	1.5 × 108	[63]
SspCA	α	9.35 × 105	1.1 × 108	[57]
SazCA	α	4.40 × 106	3.5 × 108	[61]
TaCA	α	1.60 × 106	1.6 × 108	[62]
PmCA	α	3.2 × 105	3.0 × 107	[46]
Cab	β	1.7 × 104	5.9 × 106	[64]
MtCam (expressed in E. coli and purified aerobically)	γ	6.8 × 104	3.1 × 106	[65]
MtCam (expressed in E. coli and purified anaerobically)	γ	24.3 × 104	5.4 × 106	[65]
MtCam (expressed in M. acetivorans and purified anaerobically)	γ	23.1 × 104	3.9 × 106	[66]