Table 5.
qPCR validation of the randomly selected genes.
| Item | Up-Regulated Genes | Down-Regulated Genes | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene | GnRHRA | CDKL1 | CKb | AHCY | TGFβ2 | Scp1 | ARHGEF3 | GDF7 | Wnt11 | Cyp27A |
| RNA-Seq a | 4.7826 | 4.0978 | 2.2225 | 5.0550 | 3.3047 | 11.6712 | −6.5640 | −6.9797 | −2.9406 | −5.1742 |
| qPCR b | 5.2700 | 3.9425 | 2.7950 | 6.4900 | 3.2950 | 13.8625 | −7.4600 | −8.1300 | −3.1900 | −4.4075 |
a log2 Fold Changes of DEGs between diploid and tetraploid loaches by RNA-Seq. Diploid loach was used as control; b log2 Fold Changes of DEGs between diploid and tetraploid loaches by qPCR. β-actin was used as reference gene. Gene abbreviations: Gonadotropin releasing hormone receptor type A (GnRHRA); Cyclin-dependent kinase-like 1 (CDKL1); Creatine kinase B-type (CKb); Adenosylhomocysteinase (AHCY); Transforming growth factor beta-2 (TGFβ2); Synaptonemal complex protein 1 (Scp1); Rho guanine nucleotide exchange factor (GEF) 3 (ARHGEF3); Growth/differentiation factor 7 (GDF7); Protein Wnt-11 (Wnt11) and Vitamin D3-25 hydroxylase (Cyp27A).