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. 2015 Jul 14;16(7):16017–16033. doi: 10.3390/ijms160716017

Table 5.

qPCR validation of the randomly selected genes.

Item Up-Regulated Genes Down-Regulated Genes
Gene GnRHRA CDKL1 CKb AHCY TGFβ2 Scp1 ARHGEF3 GDF7 Wnt11 Cyp27A
RNA-Seq a 4.7826 4.0978 2.2225 5.0550 3.3047 11.6712 −6.5640 −6.9797 −2.9406 −5.1742
qPCR b 5.2700 3.9425 2.7950 6.4900 3.2950 13.8625 −7.4600 −8.1300 −3.1900 −4.4075

a log2 Fold Changes of DEGs between diploid and tetraploid loaches by RNA-Seq. Diploid loach was used as control; b log2 Fold Changes of DEGs between diploid and tetraploid loaches by qPCR. β-actin was used as reference gene. Gene abbreviations: Gonadotropin releasing hormone receptor type A (GnRHRA); Cyclin-dependent kinase-like 1 (CDKL1); Creatine kinase B-type (CKb); Adenosylhomocysteinase (AHCY); Transforming growth factor beta-2 (TGFβ2); Synaptonemal complex protein 1 (Scp1); Rho guanine nucleotide exchange factor (GEF) 3 (ARHGEF3); Growth/differentiation factor 7 (GDF7); Protein Wnt-11 (Wnt11) and Vitamin D3-25 hydroxylase (Cyp27A).