Table 3.
Comparison of S. mansoni- and S. haematobium-specific real-time PCR-HRM according to eggs categorised by number of egg counts.
| Egg Count Category | No. | No. (%) Real-Time PCR-HRM +ve | Ct Value | |
|---|---|---|---|---|
| IQR | Median | |||
| Schistosoma mansoni | ||||
| Negative | 363 | 2 (0.6) | 33.0–36.7 | 35.3 |
| Light (1–99 EPG) | 19 | 19 (100) | 32.0–35.1 | 33.8 |
| Moderate (100–399 EPG) | 15 | 15 (100) | 26.9–31.4 | 29.8 |
| Heavy (≥400 EPG) | 3 | 3 (100) | 23.9–25.3 | 24.7 |
| Schistosoma haematobium | ||||
| Negative | 305 | 4 (1.3) | 29.2–34.0 | 31.7 |
| Light (1–50 EP10 mL) | 74 | 74 (100) | 25.1–33.9 | 27.5 |
| Heavy (>50 EP10 mL) | 21 | 21 (100) | 20.0–22.3 | 20.9 |
EPG, egg count per gram faeces; EP10 mL, egg count per 10 mL urine; +ve, positive; Ct, cycle threshold; IQR, Interquartile range. The median and IQR Ct values were calculated for positive real-time PCR-HRM samples per egg count category.