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. 2015 Jul 17;16(7):16242–16262. doi: 10.3390/ijms160716242

Figure 6.

Figure 6

GHR is a target of miR-146b-3p in chickens. (A) The mRNA expression of c-GHR is significantly reduced after miR-146b-3p transfection in DF-1; (B) The mRNA expression of c-GHR is significantly increased after miR-146b-3p inhibitor transfection in DF-1; (C) DF-1 cells were transfected with miR-146b-3p mimic and co-transfected with no-insert control; c-GHR wild-type or mutant luciferase reporters. The predicted binding site and mutated site of miR-146b-3p in c-GHR is shown. All of the results are expressed as the mean ± S.E.M. of three replicates. * p < 0.05; ** p < 0.01; a,b p < 0.05. WT indicates c-GHR wild-type luciferase reporter and Mut indicates c-GHR mutant luciferase reporter.