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. 2015 Jul 22;16(7):16622–16641. doi: 10.3390/ijms160716622

Figure 4.

Figure 4

Effects of 14-3-3γ overexpression on cytokine mRNA expression in LPS-induced DCMECs. DCMECs were pre-transfected with pGCMV-IRES-EGFP-14-3-3γ or pGCMV-IRES-EGFP. After 24 h, the cells were stimulated with LPS (1 μg/mL) for 6 h. The mRNA expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) were measured by RT-PCR. (A) TNF-α expression; (B) IL-6 expression; (C) IL-1β expression; (D) iNOS expression. All of these expressions were normalized to a housekeeping gene (β-actin). Bars indicate the mean ± SD (n = 3). * p < 0.05 and ** p < 0.01 vs. LPS group; ## p < 0.01 vs. control group.