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. 2015 Jul 22;16(7):16622–16641. doi: 10.3390/ijms160716622

Figure 9.

Figure 9

Effects of 14-3-3γ overexpression on lactation-associated proteins in LPS-induced DCMECs. DCMECs were pre-transfected with pGCMV-IRES-EGFP-14-3-3γ or pGCMV-IRES-EGFP. After 24 h, the cells were stimulated with LPS (1 μg/mL) for 24 h. Cytosolic proteins were analyzed by western blot for β-casein, mammalian target of rapamycin (mTOR), p-mTOR, ribosomal protein S6 kinase 1 (S6K1), p-S6K1, serine/threonine protein kinase Akt 1 (AKT1), p-AKT1, peroxisome proliferator activated receptor gamma (PPARγ), sterol regulatory element binding protein 1 (SREBP-1) and glucose transporter 1 (GLUT1). β-Actin was used as a control. Bars indicate the mean ± SD (n = 3). * p < 0.05 and ** p < 0.01 vs. LPS group; # p < 0.05 and ## p < 0.01 vs. control group.