Figure 4.

Autophagy inhibition study. (A) 3-Methyladenine (3-MA, an autophagy inhibitor) significantly attenuated fluoxetine-induced suppression of ASC viability; (B) 3-MA down-regulated the fluoxetine-induced effects on the protein levels of SQSTM1 and LC3B detected by western blotting; (C,D) 3-MA significantly down-regulated the mRNA levels of the autophagy-related genes, beclin-1 (C) and Atg7 (D), detected by quantitative real-time PCR. All values are presented as a mean ± STD (n = 3). ** p < 0.01.