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. 2015 Jul 22;16(7):16655–16668. doi: 10.3390/ijms160716655

Figure 5.

Figure 5

Fluoxetine decreased the adipogenic differentiation of ASCs. (A) ASCs were cultured in adipocyte induction medium for two weeks, stained using Oil Red O, and microscopic images are taken, 40×; (B) Fluoxetine significantly reduced the lipid accumulation in adipocyte induction medium; (C) Fluoxetine decreased the levels of adipogenic differentiation markers, peroxisome proliferator-activated receptor-γ (PPAR-γ) at day 7, but the autophagy inhibitor 3-MA significantly attenuated the mRNA levels of PPAR-γ; (D) Fluoxetine decreased the levels of adipogenic differentiation markers, CCAAT-enhancer-binding proteins-α (C/EBP-α) at day 7, but 3-MA significantly reversed the effect of fluoxetine on C/EBP-α mRNA levels, as detected by quantitative real-time PCR. GAPDH was used for normalization. All values are presented as a mean ± STD (n = 3). ** p < 0.01.