Table 1.

Purification of isocitrate lyase enzyme from recombinant Escherichia coli.

Purification Steps	Soluble Protein (mg)	Total Activity (U)	Specific Activity (U/mg)
Cell-free extract	45.2	36.2	0.8
Filtered on Ni-NTA column	2.3	3.0	1.3
Purified ICL protein	0.04	0.1	2.1