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. 2015 Jul 30;5:12720. doi: 10.1038/srep12720

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Copyright © 2015, Macmillan Publishers Limited

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Haploid h^– pat1-114 cells were cultured in nitrogen-free medium (EMM2-N) overnight at 26 °C. After 20 h, the culture was shifted to 34 °C to induce meiosis. (A) Flow cytometry of DNA contents during meiosis. Cells were sampled at the indicated time points for analysis. (B) Histone acetylation during meiosis. The cell extracts were prepared at the indicated time points and analyzed by immunoblotting. Actin was used as a loading control. Asynchronous cells (as) were obtained from preculture in YES medium.