Fig. 1.

Increased KLF12 expression was associated with impaired implantation in vivo. a The protocol for detecting the effect of KLF12 on embryo implantation. For specific details, refer to the Methods section (Animals and treatments). b The number of implantation sites (ISs) in the afternoon on 4.5 dpc in mice infected with adenovirus. c The distinctive banding pattern of a uterine horn stained with Chicago Sky Blue 6B on 4.5 dpc is indicative of an IS in mice treated with Ad-LacZ (top) and Ad-Flag-KLF12 (bottom). The arrows indicate the locations of the ISs. d A representative Western blot of Flag-tag protein in the uteri of the Ad-LacZ-treated (lanes 1–4) and Ad-Flag-KLF12-treated (lanes 5–8) mice. GAPDH was used as a loading control