Fig. 3.

Elevated KLF12 level led to decreased FOXO1 expression. hESCs were infected with adenovirus expressing either LacZ or KLF12 for 48 h at the indicated MOI. The KLF12 transcript copy number (a) and its protein expression (b) were determined by real-time PCR and Western blotting (n = 3). ***P < 0.001 compared with Ad-LacZ alone. hESCs were infected with Ad-LacZ or Ad-Flag-KLF12 at the indicated MOI for 48 h and were then treated with 8-Br-cAMP and MPA for another 2 days; subsequently, FOXO1 mRNA (c) and protein (d) levels were measured by real-time PCR and Western blotting, respectively (n = 3). *P < 0.05; **P < 0.01; and ***P < 0.001