Figure 6.

Peritubular capillary blood flow of the kidney surface is estimated with multiphoton-intravital microscopy under administration of an EET antagonist. (A) Red signals indicate peritubular capillaries, whereas green signals indicate intrinsic fluorescence of tubular cells. (B) The vascular volume percentage at 15 minutes compared from 0 minutes is significantly smaller in Mdk^−/− than in Mdk^+/+ mice. Quantitative analysis of capillary volume is carried out using Imaris software (Mdk^+/+, n=4; Mdk^−/−, n=4). *P<0.05 Mdk^+/+ versus Mdk^−/−. Data are presented as the mean±SEM.