Table 2.
Levels of each antibody in glomerular eluates and serum were determined in 20 patients with LN for whom a renal biopsy was available
| Patient | α-Enolasee | Annexin AI | H2A | H3 | H4 | DNA | C1Q | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IgG1 | IgG2 | IgG3 | IgG2 | IgG1 | IgG2 | IgG3 | IgG1 | IgG2 | IgG3 | IgG1 | IgG2 | IgG3 | IgG2 | IgG3 | IgG2 | IgG4 | |
| Ab eluted from glomeruli (ng) | |||||||||||||||||
| 1 | 0.2552 | 0.0761 | 0.1872 | 1.4884 | |||||||||||||
| 2 | 0.9197 | 0.1095 | 0.0750 | 0.6669 | |||||||||||||
| 3 | 0.5621 | ||||||||||||||||
| 4 | 2.0012 | 0.1102 | 0.0741 | 0.2493 | 0.2547 | 1.6138 | |||||||||||
| 5 | 1.3697 | 0.5005 | 0.8613 | 0.2465 | 1.4047 | ||||||||||||
| 6 | 2.0533 | 0.4996 | 0.0756 | 0.0761 | 0.0940 | ||||||||||||
| 7 | 1.2890 | 0.2585 | 0.2095 | 0.1143 | 0.1187 | 0.1543 | |||||||||||
| 8 | 0.2614 | 0.0525 | 0.1745 | ||||||||||||||
| 9 | 0.0751 | 0.0467 | 0.1279 | 0.2859 | 0.1850 | 0.2600 | |||||||||||
| 10 | 1.8999 | ||||||||||||||||
| 11 | 0.0589 | 0.2306 | 0.0740 | ||||||||||||||
| 12 | 0.1097 | 0.3023 | 0.2520 | 0.0643 | |||||||||||||
| 13 | 0.0771 | 0.2273 | 0.0538 | 0.2789 | |||||||||||||
| 14 | 0.2617 | 0.2547 | 0.2689 | ||||||||||||||
| 15 | 0.8172 | 0.0769 | 0.9267 | 0.9266 | |||||||||||||
| 16 | 0.4111 | 1.9356 | 0.0753 | 0.9899 | 0.0679 | 2.0702 | 2.0078 | 0.2510 | |||||||||
| 17 | 0.5294 | 1.5220 | 0.2098 | 0.0552 | 0.1205 | 0.1052 | |||||||||||
| 18 | 0.5287 | 1.4708 | 0.0842 | 0.0924 | |||||||||||||
| 19 | 0.5307 | 0.2150 | 0.0395 | 0.7639 | |||||||||||||
| 20 | 0.6510 | ||||||||||||||||
| Serum Ab (mg/L) | |||||||||||||||||
| 1 | 0.56 | 41.08 | 2.19 | 2.88 | 0.01 | 0.21 | 0.16 | 0.00 | 0.54 | 0.01 | 0.02 | 0.13 | 0.01 | 0.14 | 0.60 | 0.06 | 0.25 |
| 2 | 0.90 | 90.00 | 3.84 | 4.41 | 0.21 | 0.62 | 0.24 | 0.02 | 0.73 | 0.08 | 0.01 | 0.15 | 0.01 | 0.18 | 0.58 | 0.07 | 0.25 |
| 3 | 0.58 | 73.40 | 1.97 | 2.37 | 0.01 | 0.03 | 0.01 | 0.00 | 0.14 | 0.01 | 0.02 | 0.10 | 0.01 | 0.12 | 0.40 | 0.06 | 0.23 |
| 4 | 1.58 | 103.83 | 4.00 | 3.98 | 0.02 | 0.02 | 0.02 | 0.00 | 0.15 | 0.01 | 0.01 | 0.14 | 0.01 | 0.16 | 0.64 | 0.04 | 0.25 |
| 5 | 0.32 | 25.33 | 2.28 | 6.03 | 0.01 | 0.02 | 0.01 | 0.00 | 0.14 | 0.01 | 0.02 | 0.06 | 0.01 | 0.13 | 0.60 | 0.06 | 0.26 |
| 6 | 1.90 | 104.48 | 4.14 | 3.39 | 0.01 | 0.02 | 0.05 | 0.00 | 0.39 | 0.12 | 0.07 | 0.24 | 0.01 | 0.11 | 0.27 | 0.06 | 0.27 |
| 7 | 0.47 | 61.80 | 2.08 | 3.89 | 0.02 | 0.34 | 0.13 | 0.03 | 0.18 | 0.11 | 0.03 | 0.59 | 0.01 | 0.18 | 0.64 | 0.08 | 0.27 |
| 8 | 0.56 | 10.39 | 2.28 | 5.80 | 0.01 | 0.03 | 0.01 | 0.01 | 0.63 | 0.01 | 0.02 | 0.42 | 0.01 | 0.09 | 0.24 | 0.07 | 0.28 |
| 9 | 0.50 | 14.59 | 0.97 | 4.01 | 0.41 | 0.93 | 0.33 | 0.01 | 0.60 | 0.10 | 0.02 | 0.31 | 0.02 | 0.17 | 0.63 | 0.08 | 0.29 |
| 10 | 0.66 | 87.33 | 2.19 | 5.64 | 0.01 | 0.03 | 0.01 | 0.00 | 0.13 | 0.01 | 0.02 | 0.03 | 0.01 | 0.08 | 0.36 | 0.07 | 0.22 |
| 11 | 0.24 | 13.28 | 2.17 | 2.16 | 0.01 | 0.03 | 0.01 | 0.00 | 0.12 | 0.01 | 0.01 | 0.13 | 0.01 | 0.17 | 0.55 | 0.03 | 0.20 |
| 12 | 0.46 | 10.36 | 1.70 | 2.81 | 0.01 | 0.02 | 0.01 | 0.00 | 0.12 | 0.01 | 0.01 | 0.04 | 0.01 | 0.18 | 0.52 | 0.07 | 0.27 |
| 13 | 0.29 | 41.80 | 2.39 | 2.25 | 0.01 | 0.03 | 0.01 | 0.00 | 0.17 | 0.01 | 0.09 | 0.40 | 0.01 | 0.14 | 0.60 | 0.07 | 0.25 |
| 14 | 0.41 | 72.93 | 2.36 | 3.68 | 0.01 | 0.02 | 0.01 | 0.00 | 0.11 | 0.01 | 0.01 | 0.10 | 0.01 | 0.14 | 0.61 | 0.08 | 0.26 |
| 15 | 0.48 | 84.78 | 1.67 | 4.80 | 0.01 | 0.22 | 0.05 | 0.00 | 0.28 | 0.01 | 0.01 | 0.15 | 0.01 | 0.17 | 0.55 | 0.04 | 0.16 |
| 16 | 0.58 | 88.72 | 1.14 | 4.58 | 0.51 | 0.82 | 0.32 | 0.03 | 0.84 | 0.15 | 0.06 | 0.18 | 0.02 | 0.17 | 0.56 | 0.07 | 0.24 |
| 17 | 0.47 | 80.95 | 2.40 | 3.61 | 0.02 | 0.23 | 0.12 | 0.03 | 0.29 | 0.09 | 0.02 | 0.03 | 0.01 | 0.18 | 0.55 | 0.01 | 0.19 |
| 18 | 0.57 | 48.77 | 1.02 | 5.19 | 0.01 | 0.02 | 0.01 | 0.00 | 0.11 | 0.01 | 0.02 | 0.02 | 0.01 | 0.17 | 0.59 | 0.07 | 0.28 |
| 19 | 0.52 | 45.77 | 0.80 | 6.03 | 0.01 | 0.03 | 0.01 | 0.00 | 0.42 | 0.14 | 0.02 | 0.03 | 0.01 | 0.17 | 0.56 | 0.02 | 0.19 |
| 20 | 0.27 | 35.24 | 0.96 | 2.66 | 0.01 | 0.02 | 0.01 | 0.00 | 0.04 | 0.01 | 0.02 | 0.04 | 0.01 | 0.14 | 0.64 | 0.07 | 0.28 |
For serum antibody levels, specific ELISAs were used. For their concentration in glomerular eluates, a dot blot was used (Concise Methods). In some cases, levels were below the limit of detection and not reported. Results are given in nanograms per 100 μl eluate.