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. Author manuscript; available in PMC: 2015 Jul 30.
Published in final edited form as: Nat Plants. 2015 Feb 16;1(3):14030. doi: 10.1038/nplants.2014.30

Figure 1. Isolation of TIR1 mutants in yeast.

Figure 1

a, The TIR1 proteins E12K, E15K and F18L dramatically increase X-Gal staining when in combination with IAA7. b, Activity of β-galactosidase in extracts from yeast strains in a. Error bars are s.e.m. c, Levels of TIR1 and IAA7 proteins in yeast. TIR–Myc levels in extracts prepared from yeast strains in a determined by protein blot. Protein samples (20 µg) were loaded in each lane. d, Auxin-induced degradation of YFP–IAA fusion proteins in yeast in the presence of wild-type and mutant TIR1 proteins. Fluorescence levels were captured using time-lapse flow cytometry. Degradation curves were normalized to the starting fluorescence. a.u., absorbance units; U, units.