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. Author manuscript; available in PMC: 2015 Jul 30.
Published in final edited form as: Nat Plants. 2015 Feb 16;1(3):14030. doi: 10.1038/nplants.2014.30

Figure 3. The tir1E12K and E15K proteins are deficient in SCF assembly.

Figure 3

a, Co-immunoprecipitation (IP) assays performed with wild-type and mutant GUS fusion proteins. CUL1 was detected with an anti-CUL1 antibody. The tir1E12K, tir1E15K and AFB1 extracts were diluted relative to the wild-type protein so that approximately equal amounts of fusion protein was present during the IP. b, Quantification of band intensities shown in a using a chemiluminescence imager. Values are expressed relative to the wild type. Error bars are s.d. c, In vivo pull-down of GST–IAA7 from extracts prepared from lines expressing wild-type and mutant GUS fusion protein. As in a approximately equal amount of GUS fusion protein were present in each pull-down reaction.