Table 1.
TO1-2p | TO1-2p-Cy5 | TO | Coumarin-TO | |
---|---|---|---|---|
ε (M−1cm−1)a | 49,500 | 49,500 (TO1) 200,000 (Cy5) |
63,000 | 17,300 (coumarin) 49,200 (TO) |
λabs (nm)b | 504 | 511 (TO1) 650 (Cy5) |
505 | 400 (coumarin) 508 (TO) |
KD (nM)b | 0.3 | 12 | 0.17 | 1.1 |
Φb | 0.47 | 0.37 | 0.89 | 0.87 |
FRET%b | N/A | 96% | N/A | 99% |
Determined in MeOH.
Determined in the presence of cognate FAP.