Fig. 2.

Representative fluorescence images from pig mammary organoids 48 h after dissociation (P0) and after passages 1 and 2 (P1 and P2). Four distinct populations of cells were visible at P0. Cytokeratin-positive luminal epithelial cells, vimentin-positive fibroblasts (dashed circle, arrowheads in P2), cells positive for both vimentin and cytokeratin (dashed rectangle) and small, vimentin- positive cells (solid circle) found infrequently only at P0