Figure 5.

Alternative analysis of the Grp1_63–399 SEC–SAXS data. (a) Transposed scattering data fitted with a sum of exponentially modified Gaussians as described in the text. (b) Individual components from a fit of the total scattering profile [equivalent to a summation of the transposed data in (a)] with a sum of exponentially modified Gaussians, where b is a baseline constant and eg1, eg2 and eg3 are the individual amplitude-weighted exponential Gaussian terms. (c) Comparison of the fitted baseline constant b(q) and the raw scattering data. Note that b(q) strongly resembles the expected buffer scattering. (d) R ² values for Guinier optimization of the scaling constant for buffer subtraction using b(q) as the buffer. Comparison of protein scattering curves (e) and weighted Guinier fits (f) for direct and Guinier-optimized buffer subtraction using b(q) as buffer with the protein scattering curves and Guinier fits for Guinier-optimized reconstruction following SVD. For comparison, the curves were scaled by linear least squares.