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. 2015 Jul 8;48(Pt 4):1102–1113. doi: 10.1107/S1600576715010420

Figure 6.

Figure 6

Conventional SAXS experiments for Arf6 and Grp1 after SEC. SAXS data sets for Arf6NΔ13Q67L (a) and Grp163–399 (b) acquired after chromatography on a 3 ml Superdex-200 Increase column. Individual curves are from consecutive acquisitions under flow. (c) Fit of the His6-Arf6NΔ13Q67L CRYSOL model to the direct and Guinier-optimized buffer subtractions after averaging the data in (a). (d) Fit of the His6-Grp163–399 chain A monomer CRYSOL model to the direct and Guinier-optimized buffer subtractions after averaging the data in (b). Fits of the subtractions in (d) to a linear combination of CRYSOL models for the His6-Grp163–399 chain A monomer and chain A/B dimer either without (e) or including (f) a buffer scattering component. Black lines represent fitted models. All fits were performed by general linear least squares in DELA using theoretical models from CRYSOL.