Quantification by RT-qPCR of Livin expression in mouse tissue. (A) In 1-month-old mice, Livin is strongly expressed in lens compared with other tissues (liver, lung, retina) examined. (B) Livin expression in the lens commences between E12.5 and E16.5. Expression increases throughout the early postnatal period, peaking at postnatal (P) day 30. Although expression levels are somewhat reduced in older animals, Livin is an abundant transcript at all ages. (C) Within the lens, Livin expression is undetectable in the epithelium, and strongest in the inner cortical fiber cells. Data represent mean and standard deviation of at least three determinations in each case.