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. 2015 Apr 20;25(4):391–400. doi: 10.1111/bpa.12161

Figure 2.

figure

Loss of Sparc increases p53‐null astrocyte proliferation but suppresses growth in soft agar. A. p53‐null/Sparc‐wt (Ast11.9) and p53‐null/Sparc‐null (#2, #11 and #30) astrocytes were analyzed for proliferation at 0–5 days timepoints by measuring dsDNA content using a fluorescent assay. The assay was repeated four times and analyzed for differences in growth rate between the clones. Mean ± SEM per day are presented here. Based on the daily fold change in cell proliferation, there is a significant increase in growth for the Sparc‐null clones relative to Ast11.9 (* = P < 0.01; ** = P < 0.0001). B. Astrocytes were grown in soft agar and imaged after 10 days. Representative images show a reduction in colony‐forming ability for the Sparc‐null clones #11 and #30 (inset) compared with the Sparc‐wt cell lines Ast11.9 and Ast11.9‐2 and the glioma cell line U87MG.