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. Author manuscript; available in PMC: 2015 Jul 30.
Published in final edited form as: Mol Immunol. 2013 Oct 26;57(2):191–199. doi: 10.1016/j.molimm.2013.09.007

Figure 1. Hp91 forms spontaneous, reversible dimers.

Figure 1

(A–B) Hp91 peptide was dissolved in PBS and incubated at RT in the presence of ambient oxygen for up to 96 h. Peptides were analyzed by HPLC. Peptide monomers show a peak at an earlier time point than the peptide dimers (12.2 min vs. 12.7 min respectively). Percent dimer was determined by measuring the area under the curve (AUC) and calculating the dimer AUC/total AUC. Results shown are (A) representative of several independent experiments and (B) mean (±SEM) for n=3–4. (C) Peptides incubated with Hp91 for 24 hours were subsequently incubated +/− 10 mM DTT for 30 minutes prior to analysis by HPLC. DTT incubated sample offset on graph for ease of viewing. Results are representative of two independent experiments.