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. 2015 Jul 30;10(7):e0134013. doi: 10.1371/journal.pone.0134013

Fig 7. MAML1, CDK8 and p300 cooperatively stimulates Notch activity.

Fig 7

(A) HEK293 cells were co-transfected with a GAL4 luciferase gene and vectors expressing GAL4-Notch, MAML1, p300 and CDK8, as indicated in the figure. The data is presented as mean ± SD (n = 3). MAML1, p300, and CDK8 stimulate transcription in the presence of GAL4-Notch (*, p<0.0001). (B) Schematic of the in vitro transcription assay using chromatin templates. (C) Chromatin templates containing CSL binding sites were incubated with N1ICD, MAML1, CDK8, p300 and acetyl-CoA, as indicated.