Skip to main content
. 2015 Jul 30;10(7):e0133373. doi: 10.1371/journal.pone.0133373

Fig 4. fdCas9 exhibited robust activity with high target specificity.

Fig 4

The genome modification activities of fdCas9 were assayed at known Cas9 off-targets. (A) T7EI assays at HBD, a known off-target for the HBB genomic target, showed high modification frequencies using wtCas9 with various gRNAs targeting HBB. (B) T7EI assays at HBD showed no detectable modification activities using fdCas9. (C) T7EI assays at CCR2, a known off-target for the CCR5 genomic target showed high modification frequencies with wtCas9 using various gRNAs targeting CCR5. (D) T7EI assays at HBD showed no detectable modification using fdCas9 guided by different combinations of gRNAs.