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. 2015 Jul 30;10(7):e0133373. doi: 10.1371/journal.pone.0133373

Fig 5. fdCas9 exhibited significantly improved specificity.

Fig 5

T7EI assays to determine the catalytic activity of fdCas9 on potential off-targets, identified by PROGNOS web-tool, for EMX1, AAVS1, CCR5 and HBB genomic targets. T7EI mutation detection assays for potential off-targets of EMX1.4 and EMX1.14 gRNA pair (Fig 5A). T7EI mutation detection assays for potential off-targets of AAVS1.2 and AAVS1.10 gRNA pair (Fig 5B). T7EI mutation detection assays for potential off-targets of CCR5.13 and CCR5.15 gRNA pair (Fig 5C). T7EI mutation detection assays for potential off-targets of HBB9 and HBB10 gRNA pair (Fig 5D). Note: * indicates the expected size of the DNA bands of corresponding amplicons cleaved by T7EI.