Table 3. Spearman correlation analysis between OTU M7and OTUs showing the largest (top 20) changes in relative abundance between rumen liquid of group R and group A.

Taxon	OTU number	FDR	LR average	LA average	LR-LA	Spearman ρ with M7	Spearman P with M7
Succinivibrionaceae	3004	0.0005	0.100	8.755	-8.65	-0.72	<1X10-20
Prevotella	649	0.0009	0.092	4.930	-4.84	-0.24	NS
Prevotella	570	0.0005	0.029	2.882	-2.85	-0.10	NS
Prevotella	595	0.0006	0.053	2.270	-2.22	-0.17	NS
Clostridiales	1130	0.0005	0.056	1.760	-1.70	-0.12	NS
Prevotella	645	0.0014	0.066	1.709	-1.64	-0.01	NS
Prevotella	555	0.0005	0.016	1.566	-1.55	-0.01	NS
Prevotella	548	0.0005	0.013	1.456	-1.44	-0.08	NS
Prevotella	608	0.0005	0.010	1.195	-1.18	0.27	0.047
Lachnospiraceae	1823	0.00046	0.011	1.141	-1.13	-0.12	NS
Bifidobacterium	44	0.0074	1.165	0.014	1.15	0.27	0.048
Butyrivibrio	2052	0.0005	1.226	0.073	1.15	-0.21	NS
Succiniclasticum	2753	0.0050	2.155	0.718	1.44	-0.05	NS
Ruminococcus	2598	0.0046	1.910	0.449	1.46	-0.04	NS
Bacteroidales	203	0.0005	1.643	0.134	1.51	-0.28	NS
Prevotella	566	0.0011	2.382	0.667	1.71	0.19	NS
Ruminococcus	2625	0.0006	2.001	0.161	1.84	0.23	NS
Prevotella	571	0.0005	1.921	0.071	1.85	0.05	NS
Butyrivibrio	2050	0.0005	2.487	0.186	2.30	0.07	NS
Methanobrevibacter	7	0.0031	4.824	1.529	3.30	1.00	0.000

FDR = False discovery rate of change in relative abundance between group R and group A. LR average = average % relative abundance in the liquid fraction of group R. LA average = average % relative abundance in the liquid fraction of group A. LR-LA = LA average subtracted from LR average. Spearman ρ and Spearman P with M7 = Spearman’s rank correlation coefficient with relative abundance of OTU-M7 and corresponding P value. NS = non-significant (i.e. P > 0.05).