Table 4. Spearman correlation analysis between OTU M7and OTUs showing the largest (top 20) changes in relative abundance between rumen solid of group R and group A.

Taxon	OTU number	FDR	SR average	SA average	SR-SA	Spearman ρ with M7	Spearman P with M7
Succinivibrionaceae	3004	0.0004	0.008	3.438	-3.43	-0.24	0.08
Prevotella	570	0.0004	0.003	3.071	-3.07	-0.14	NS
Prevotella	608	0.0004	0.002	2.845	-2.84	-0.23	0.09
Prevotella	649	0.0014	0.022	2.380	-2.36	-0.08	NS
Megasphaera	2729	0.0155	1.088	3.315	-2.23	-0.12	NS
Clostridiales	1130	0.0009	0.048	2.126	-2.08	-0.04	NS
Prevotella	595	0.0007	0.020	1.684	-1.66	-0.16	NS
Succiniclasticum	2761	0.0010	0.001	1.551	-1.55	0.09	NS
Prevotella	645	0.0062	0.031	1.521	-1.49	0.12	NS
Lachnospiraceae	1831	0.0010	0.002	1.428	-1.43	-0.04	NS
Butyrivibrio	2075	0.0016	0.814	0.170	0.64	-0.05	NS
Ruminococcus	2598	0.0027	1.168	0.251	0.92	-0.08	NS
Ruminococcus	2594	0.0009	1.158	0.133	1.02	-0.08	NS
Clostridiales	1156	0.0031	1.361	0.322	1.04	0.09	NS
Butyrivibrio	2052	0.0004	1.203	0.091	1.11	-0.23	0.09
Bifidobacterium	44	0.0007	1.212	0.028	1.18	0.09	NS
Ruminococcus	2625	0.0006	1.612	0.103	1.51	0.00	NS
Bacteroidales	203	0.0009	1.997	0.230	1.77	0.02	NS
Methanobrevibacter	7	0.0011	2.686	0.545	2.14	1.00	0.000
Butyrivibrio	2050	0.0006	2.424	0.233	2.19	0.01	NS

FDR = False discovery rate of change in relative abundance between group R and group A. SR average = average % relative abundance in the solid fraction of group R. SA average = average % relative abundance in the solid fraction of group A. SR-SA = SA average subtracted from SR average. Spearman ρ and Spearman P with M7 = Spearman’s rank correlation coefficient with relative abundance of OTU-M7 and corresponding P value. NS = non-significant (i.e. P > 0.05).