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. 2015 Jul 30;10(7):e0134003. doi: 10.1371/journal.pone.0134003

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© 2015 Namachivayam et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — A. Western blots show the genome-wide acetylation status of histones H2A, H2B, H3, and H4. Bar-diagram (means ± SE) summarizes densitometric data normalized against β-actin. B. AtRA treatment increases the acetylation of histone H2B (lys5) on the TGF-β₂ nucleosome. Bar diagram (means ± SE) shows data from ChIP assay, where the acetylated histones were pulled down and the presence of the TGF-β₂ promoter region in the complex confirmed by real-time PCR. Quantification of acetyl-H2B is shown as % input. Inset: Agarose gel showing enrichment of acetyl-H2B in the TGF-β₂ promoter. Data represent 3 separate experiments; * p<0.05, *** p<0.001.