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Effect of the Na-Cl cotransporter (NCC) blocker hydrochlorothiazide (HCTZ) on PSF when NKCC2 and CTGF are inhibited. Left: in the presence of furosemide and benzamil (○), adding the NCC inhibitor HCTZ (●) did not affect PSF, suggesting that the distal convoluted tubule does not participate in the regulation of Af-Art tone. Right: maximum PSF responses in furosemide+benzamil and furosemide+benzamil+HCTZ curves.
