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. 2015 Jul 30;10(7):e0134299. doi: 10.1371/journal.pone.0134299

Fig 4. Enhanced apoptosis in proliferation regions of the ddb1 m863 mutant CNS.

Fig 4

(A-F) The pcna expression pattern in ddb1 m863 mutants and wild type siblings at 30 hpf (A-B) and 72 hpf (C-F). (G-J) TUNEL assay for apoptosis in ddb1 m863 mutants (H-H', J) and wild type siblings (G-G', I) at 72 hpf. Embryos or larvae in lateral (A-B, E-H) and ventral views (C-D). Cross sections (20 μm; I-J) from larvae by TUNEL assay. Arrows represent affected pcna-expressing cells or enhanced apoptotic cells in the retina (D, J; red), pretectal region (F, J; blue), and cerebellum (F; green). Abbreviations used: ba, branchial arches; CMZ, ciliary marginal zone; Cce, cerebellum; GCL, ganglion cell layer; hb, hindbrain; H, hypothalamus; INL, inner nuclear layer; int, intestine; P, pallial proliferation zone; PO, preoptic area; tpz, tectal proliferation zones; Anterior towards the left. Scale bars in H' for G' and H', in J for all of others: 100 μm.