Table 1. Dopaminergic phenotype in ddb1_MO and p53_MO knockdown larvae.

	Morpholinos		Embryos	Phenotypic class
	ddb1 MOs	p53_MO	Total No.	I	II	IIIa	IIIb	IV
ddb1_MO1	4ng	0ng	39	8	7	10	14	0
	4ng	4ng	62	7	13	17	25	0
	6ng	0ng	16	1	0	3	12	0
	6ng	6ng	51	1	5	25	17	3
	8ng	0ng	39	0	1	0	38	0
	8ng	7ng	28	0	0	10	18	0
ddb1_MO2	6ng	0ng	29	3	12	14	0	0
	6ng	6ng	31	2	14	15	0	0
	8ng	0ng	25	0	0	4	19	2
	8ng	7ng	38	3	0	11	21	3

Summary of results from ddb1 morpholino knockdown experiments. Amounts of morpholino injected per embryo are given in ng. Injected embryos were allowed to develop until 72 hpf, fixed, and stained by WISH for th expression. Zebrafish larvae from each knockdown experiment were classified into different phenotypic classes according to the effect of the knockdown on the th expressing pattern and on morphology. I, th expression pattern was similar to wild type siblings; II, th expressing cells were slightly reduced in number; IIIa, th expressing cell number was reduced in the pretectum and retina; IIIb, th-expressing cell number clearly reduced plus decreased size of brain and/or retina; IV, dying and decaying embryos. Based on the changes in th-expression pattern, the class IIIa/b phenotype was similar to that of m863 mutants.