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. 2015 Jul 30;10(7):e0134411. doi: 10.1371/journal.pone.0134411

Fig 2. Characterization of the cell line expressing the DDR-Act-FP reporter.

Fig 2

HCT116 cells stably expressing the DDR-Act reporter were left untreated (NT) or treated with Nutlin-3 (Nut 20 μM), or with Etoposide concentrations from 0.5 to 20 μM (E0.5 to E20) for 24 hours. (A) Western blot analysis of p53, γH2AX and actin level. (B) Bar chart represents the average fluorescence intensity+/-SD monitored after 24h. 1500 cells from 3 independent wells were analyzed for each condition using the Cellomics scan software. *:P<0.05; **:P<0.01; ***:P<0.005 (One-way ANOVA, Prism).