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. 2015 Jul 30;10(7):e0134411. doi: 10.1371/journal.pone.0134411

Fig 3. Pharmacological manipulation of the DDR-Act-FP reporter using ATM kinase inhibitors.

Fig 3

HCT116 cells expressing the DDR-Act reporter were treated with Etoposide 20 μM (E20) for 24 hours, in the absence or in the presence of KU-55933 or CP-466722 at the indicated concentration. (A) Western blot analysis of p53 and actin as control. (B) Fluorescence level was monitored after 24h. Bar graph represents the average fluorescence intensity+/-SD from 6 samples (500 cells analysed/sample with Cellomics scan software) for each condition. *:P<0.05; **:P<0.01; ***:P<0.005 (Unpaired t-test, Prism).