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. 2015 Jul 30;10(7):e0134595. doi: 10.1371/journal.pone.0134595

Fig 2. Quantitative RT-PCR validation of RNA-sequencing results.

Fig 2

(A) Quantitative RT-PCR analysis of the mRNA expression of genes differentially expressed after treatment with different levels of H2O2. M. smegmatis cultures were treated with 2 mM or 7 mM H2O2 for 30 min before extraction of RNA for qRT-PCR. The data represent 3 independent experiments. (B) Fold changes of selected genes differentially expressed genes after treatment with 0.2 mM and 7 mM H2O2 obtained by the RNA-sequencing.