Table 1. Thermodynamic Parameters for the Binding of Carbohydrates and (Glyco)peptides to Galectin-3 (full-length and CRD) Determined by ITCa.
| Ka (×10–4 M–1) | –ΔG (kcal/mol) | –ΔH (kcal/mol) | –TΔS (kcal/mol) | n | Kd (μM) | |
|---|---|---|---|---|---|---|
| Galectin-3 | ||||||
| LacNAc | 3.06 | 6.14 | 12.74 | 6.60 | 0.94 | 33 |
| TF | 0.40 | 5.44 | 8.74 | 3.30 | 0.82 | 245 |
| Thr-TF | 0.35 | 4.85 | 8.78 | 3.93 | 1.02 | 283 |
| MUC1 | no binding | – | – | – | – | – |
| MUC1-Thr4 | 3.60 | 6.26 | 0.56 | –5.70 | 0.76 | 28 |
| MUC1-Thr9 | 2.22 | 5.95 | 1.75 | –4.20 | 1.03 | 45 |
| MUC1-Thr16 | 2.21 | 5.93 | 0.73 | –5.20 | 0.86 | 45 |
| MUC1-Ser5 | 1.60 | 5.74 | 1.33 | –4.41 | 0.70 | 63 |
| MUC11–15-Thr1 | 0.76 | 5.29 | 1.79 | –3.50 | 0.87 | 132 |
| MUC11–8-Thr4 | 0.29 | 4.72 | 1.32 | –3.40 | 0.97 | 346 |
| Galectin-3 CRD | ||||||
| LacNAc | 3.31 | 6.10 | 13.70 | 7.60 | 1.08 | 30 |
| TF | 0.40 | 4.94 | 14.05 | 9.11 | 0.93 | 248 |
| Thr-TF | 0.37 | 4.88 | 12.63 | 7.75 | 0.90 | 272 |
| MUC1-Thr4 | 1.06 | 5.46 | 0.56 | –4.90 | 0.80 | 94 |
| MUC1-Thr9 | 0.97 | 5.44 | 0.94 | –4.50 | 0.98 | 103 |
| MUC1-Ser5 | 1.00 | 5.44 | 4.11 | –1.34 | 0.92 | 103 |
a
The binding isotherms are presented in Figures 2 and 3 and Figures S15 and S16 of the Supporting Information.